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JKFN Journal of the Korean Society of Food Science and Nutrition



Online ISSN 2288-5978

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Journal of the Korean Society of Food Science and Nutrition 2020; 49(7): 659-667

Published online July 31, 2020

Copyright © The Korean Society of Food Science and Nutrition.

Inhibitory Effect of the Extract of Cheonggukjang Fermented with Bacillus amyloliquefaciens SCGB 1 on LPS-Induced Inflammation and Inflammatory Diseases

So Yeon Kim1, Kyung Bok Lee2,3, Young Ho Cho4, Do Youn Jeong5, Hee Jong Yang5, Myeong Seon Ryu5, and Yung Choon Yoo1,3

1Department of Microbiology, College of Medicine, 2Department of Biochemistry, College of Medicine, 3Myunggok Medical Research Institute, and 4Department of Pharmaceutics & Biotechnology, College of Medical Engineering, Konyang University 5Microbial

Abstract

Cheonggukjang is a Korean fermented food. Cheonggukjang has many biological activities such as anticancer and antioxidant effects. In this study, we investigated the anti-inflammatory effect of the hot water extract (SCGB 1) of cheonggukjang fermented with Bacillus amyloliquefaciens SCGB 1 (KCCM11964P) on lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. We examined the effect of the cheonggukjang extract on septicemia and gastritis induced by LPS and HCl/EtOH, respectively in mice. Treatment of SCGB 1 significantly inhibited inflammatory mediators such as nitric oxide (NO), tumor-necrosis factor (TNF)-α, and interleukin-6 (IL-6) in LPS-stimulated RAW 264.7 cells. Its anti-inflammatory effect was confirmed by inhibition of the expression of mRNAs of inducible NO synthase, TNF-α, and IL-6. In addition, SCGB 1 inhibited activation of mitogen-activated protein kinases (MAPK) and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB). In an in vivo experiment, oral administration of SCGB 1 significantly suppressed the lethal septic shock induced by LPS injection in mice. Furthermore, mice given SCGB 1 showed significant inhibition of severe gastritis induced by HCl/EtOH in mice. Taken together, SCGB 1 is an excellent material to inhibit inflammation and inflammatory diseases.

Keywords: B. amyloliquefaciens SCGB 1, anti-inflammatory activity, sepsis, gastritis, signal transduction

Article

Article

Journal of the Korean Society of Food Science and Nutrition 2020; 49(7): 659-667

Published online July 31, 2020

Copyright © The Korean Society of Food Science and Nutrition.

Bacillus amyloliquefaciens SCGB 1 발효 청국장 추출물에 의한 항염증 활성 및 염증성 질환 억제 효과

Inhibitory Effect of the Extract of Cheonggukjang Fermented with Bacillus amyloliquefaciens SCGB 1 on LPS-Induced Inflammation and Inflammatory Diseases

So Yeon Kim*1**, Kyung Bok Lee*2*,*3**, Young Ho Cho*4, Do Youn Jeong*5, Hee Jong Yang*5, Myeong Seon Ryu*5, and Yung Choon Yoo*1*,*3

*1Department of Microbiology, College of Medicine, *2Department of Biochemistry, College of Medicine, *3Myunggok Medical Research Institute, and *4Department of Pharmaceutics & Biotechnology, College of Medical Engineering, Konyang University *5Microbial

Abstract

Cheonggukjang is a Korean fermented food. Cheonggukjang has many biological activities such as anticancer and antioxidant effects. In this study, we investigated the anti-inflammatory effect of the hot water extract (SCGB 1) of cheonggukjang fermented with Bacillus amyloliquefaciens SCGB 1 (KCCM11964P) on lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. We examined the effect of the cheonggukjang extract on septicemia and gastritis induced by LPS and HCl/EtOH, respectively in mice. Treatment of SCGB 1 significantly inhibited inflammatory mediators such as nitric oxide (NO), tumor-necrosis factor (TNF)-α, and interleukin-6 (IL-6) in LPS-stimulated RAW 264.7 cells. Its anti-inflammatory effect was confirmed by inhibition of the expression of mRNAs of inducible NO synthase, TNF-α, and IL-6. In addition, SCGB 1 inhibited activation of mitogen-activated protein kinases (MAPK) and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB). In an in vivo experiment, oral administration of SCGB 1 significantly suppressed the lethal septic shock induced by LPS injection in mice. Furthermore, mice given SCGB 1 showed significant inhibition of severe gastritis induced by HCl/EtOH in mice. Taken together, SCGB 1 is an excellent material to inhibit inflammation and inflammatory diseases.

Keywords: B. amyloliquefaciens SCGB 1, anti-inflammatory activity, sepsis, gastritis, signal transduction