Many studies have been conducted to increase the content of ergocalciferol (vitamin D) in mushrooms. This study established the optimal conditions for ergocalciferol-fortified Lentinula edodes. This study investigated the effects of UV-B irradiation with micronized mushrooms for increasing ergocalciferol in five kinds of mushrooms (Pleurotus eryngii, Pleurotus ostreatus, Flammulina velutipes, Agaricus bisporus, and L. edodes). The content of ergocalciferol increased in the mushrooms irradiated to UV-B for 30 minutes. The highest ergocalciferol content (76.12±2.53 μg/g; the conversion of ergosterol to ergocalciferol: 2.98%) was observed in L. edodes irradiated with UV-B. Irradiation of micronized L. edodes for 15 minutes was found to be the optimum period of irradiation in ergocalciferol content (35.54±1.11 μg/g) and conversion (5.56%). L. edodes in Gyeongsangdo, which had been irradiated to UV-B for 15 minutes, also showed the high content (99.92±3.71 μg/g) and conversion (13.45%) to ergocalciferol.

Keywords: vitamin D, ergosterol, ergocalciferol, mushroom