Ex) Article Title, Author, Keywords
Online ISSN 2288-5978
Ex) Article Title, Author, Keywords
Journal of the Korean Society of Food Science and Nutrition 2020; 49(2): 127-133
Published online February 29, 2020
Copyright © The Korean Society of Food Science and Nutrition.
Eui-Baek Byun1, Kwangwook Kim2, Woo Sik Kim1, and Eui-Hong Byun2
1Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute; 2Department of Food Science and Technology, Kongju National University
This study focused on the macrophage protection effects of Annona muricata leaf polysaccharides (ALPS). Macrophage apoptosis was induced by a staurosporine (STS) treatment. STS induced macrophage apoptosis because of the cytotoxicity, caspase activation, reactive oxygen species (ROS) production, and increase in mitochondrial outer membrane permeability (MOMP). The inhibition of macrophage apoptosis by the ALPS treatment was confirmed. The ALPS treatment reduced STS-induced cell death and Annexin V positive cell levels significantly in a dose-dependent manner. In addition, the ALPS treatment inhibited the activation of caspase-3, 8, 9, and PARP in macrophages. Furthermore, the ALPS treatment reduced the STS-induced ROS production and MOMP increase in macrophages. Therefore, the ALPS treatment inhibits STS-induced macrophage apoptosis through the inhibition of cytotoxicity, caspase activation, ROS production, and MOMP increase, suggesting that ALPS could be a potential candidate as an immune cell protection drug.
Keywords: Annona muricata, macrophage, apoptosis, cytoprotective effects
Journal of the Korean Society of Food Science and Nutrition 2020; 49(2): 127-133
Published online February 29, 2020
Copyright © The Korean Society of Food Science and Nutrition.
Eui-Baek Byun*1, Kwangwook Kim*2, Woo Sik Kim*1, and Eui-Hong Byun*2
*1Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute; *2Department of Food Science and Technology, Kongju National University
This study focused on the macrophage protection effects of Annona muricata leaf polysaccharides (ALPS). Macrophage apoptosis was induced by a staurosporine (STS) treatment. STS induced macrophage apoptosis because of the cytotoxicity, caspase activation, reactive oxygen species (ROS) production, and increase in mitochondrial outer membrane permeability (MOMP). The inhibition of macrophage apoptosis by the ALPS treatment was confirmed. The ALPS treatment reduced STS-induced cell death and Annexin V positive cell levels significantly in a dose-dependent manner. In addition, the ALPS treatment inhibited the activation of caspase-3, 8, 9, and PARP in macrophages. Furthermore, the ALPS treatment reduced the STS-induced ROS production and MOMP increase in macrophages. Therefore, the ALPS treatment inhibits STS-induced macrophage apoptosis through the inhibition of cytotoxicity, caspase activation, ROS production, and MOMP increase, suggesting that ALPS could be a potential candidate as an immune cell protection drug.
Keywords: Annona muricata, macrophage, apoptosis, cytoprotective effects
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