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JKFN Journal of the Korean Society of Food Science and Nutrition



Online ISSN 2288-5978

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Journal of the Korean Society of Food Science and Nutrition 2020; 49(2): 127-133

Published online February 29, 2020

Copyright © The Korean Society of Food Science and Nutrition.

Cytoprotective Effects of Crude Polysaccharides Separated from Annona muricata Leaf in Macrophage

Eui-Baek Byun1, Kwangwook Kim2, Woo Sik Kim1, and Eui-Hong Byun2

1Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute; 2Department of Food Science and Technology, Kongju National University

Abstract

This study focused on the macrophage protection effects of Annona muricata leaf polysaccharides (ALPS). Macrophage apoptosis was induced by a staurosporine (STS) treatment. STS induced macrophage apoptosis because of the cytotoxicity, caspase activation, reactive oxygen species (ROS) production, and increase in mitochondrial outer membrane permeability (MOMP). The inhibition of macrophage apoptosis by the ALPS treatment was confirmed. The ALPS treatment reduced STS-induced cell death and Annexin V positive cell levels significantly in a dose-dependent manner. In addition, the ALPS treatment inhibited the activation of caspase-3, 8, 9, and PARP in macrophages. Furthermore, the ALPS treatment reduced the STS-induced ROS production and MOMP increase in macrophages. Therefore, the ALPS treatment inhibits STS-induced macrophage apoptosis through the inhibition of cytotoxicity, caspase activation, ROS production, and MOMP increase, suggesting that ALPS could be a potential candidate as an immune cell protection drug.

Keywords: Annona muricata, macrophage, apoptosis, cytoprotective effects

Article

Article

Journal of the Korean Society of Food Science and Nutrition 2020; 49(2): 127-133

Published online February 29, 2020

Copyright © The Korean Society of Food Science and Nutrition.

그라비올라(Annona muricata) 잎에서 분리된 다당류 추출물의 큰포식세포 보호 효과

Cytoprotective Effects of Crude Polysaccharides Separated from Annona muricata Leaf in Macrophage

Eui-Baek Byun*1, Kwangwook Kim*2, Woo Sik Kim*1, and Eui-Hong Byun*2

*1Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute; *2Department of Food Science and Technology, Kongju National University

Abstract

This study focused on the macrophage protection effects of Annona muricata leaf polysaccharides (ALPS). Macrophage apoptosis was induced by a staurosporine (STS) treatment. STS induced macrophage apoptosis because of the cytotoxicity, caspase activation, reactive oxygen species (ROS) production, and increase in mitochondrial outer membrane permeability (MOMP). The inhibition of macrophage apoptosis by the ALPS treatment was confirmed. The ALPS treatment reduced STS-induced cell death and Annexin V positive cell levels significantly in a dose-dependent manner. In addition, the ALPS treatment inhibited the activation of caspase-3, 8, 9, and PARP in macrophages. Furthermore, the ALPS treatment reduced the STS-induced ROS production and MOMP increase in macrophages. Therefore, the ALPS treatment inhibits STS-induced macrophage apoptosis through the inhibition of cytotoxicity, caspase activation, ROS production, and MOMP increase, suggesting that ALPS could be a potential candidate as an immune cell protection drug.

Keywords: Annona muricata, macrophage, apoptosis, cytoprotective effects