Ex) Article Title, Author, Keywords
Online ISSN 2288-5978
Ex) Article Title, Author, Keywords
Journal of the Korean Society of Food Science and Nutrition 2019; 48(12): 1330-1336
Published online December 31, 2019
Copyright © The Korean Society of Food Science and Nutrition.
Eui-Baek Byun1, Kwangwook Kim2, Nam-Soon Oh2, Won-Jong Park2, Sang-Hyun Park2, Woo Sik Kim1, Ha-Yeon Song1, Jeong Moo Han1, and Eui-Hong Byun2
1Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute; 2Department of Food Science and Technology, Kongju National University
It is believed that Cudrania tricuspidata fruits polysaccharides extracts (CTPS) have antioxidant, anti-inflammatory, anti-cancer, and immune activity effects. However, there have been no studies that have shown the cytoprotective effects of CTPS. Therefore, the present study was conducted to confirm the cytoprotective effect of CTPS in terms of their inhibition of cytotoxicity, caspase activity, reactive oxygen species (ROS) production, and mitochondrial outer membrane permeability (MOMP) increase in Raw264.7. Macrophage apoptosis was induced by staurosporine (STS) treatment. CTPS treatment significantly reduced STS induced cell death and annexin V positive cell levels in a dose-dependent manner. In addition, CTPS treatment inhibited activation of caspase-3, 8, 9, and PARP in macrophages. Further, CTPS treatment reduced STS induced ROS production and MOMP increase in macrophages. In conclusion, CTPS treatment inhibits STS-induced macrophage apoptosis through inhibition of cytotoxicity, caspase activation, ROS production, and MOMP increase. These results suggest CTPS could be a potential candidate for treatment of immune cell protection effects.
Keywords: Cudrania tricuspidata, macrophage, apoptosis, protective roles
Journal of the Korean Society of Food Science and Nutrition 2019; 48(12): 1330-1336
Published online December 31, 2019
Copyright © The Korean Society of Food Science and Nutrition.
Eui-Baek Byun*1, Kwangwook Kim*2, Nam-Soon Oh*2, Won-Jong Park*2, Sang-Hyun Park*2, Woo Sik Kim*1, Ha-Yeon Song*1, Jeong Moo Han*1, and Eui-Hong Byun*2
*1Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute; *2Department of Food Science and Technology, Kongju National University
It is believed that Cudrania tricuspidata fruits polysaccharides extracts (CTPS) have antioxidant, anti-inflammatory, anti-cancer, and immune activity effects. However, there have been no studies that have shown the cytoprotective effects of CTPS. Therefore, the present study was conducted to confirm the cytoprotective effect of CTPS in terms of their inhibition of cytotoxicity, caspase activity, reactive oxygen species (ROS) production, and mitochondrial outer membrane permeability (MOMP) increase in Raw264.7. Macrophage apoptosis was induced by staurosporine (STS) treatment. CTPS treatment significantly reduced STS induced cell death and annexin V positive cell levels in a dose-dependent manner. In addition, CTPS treatment inhibited activation of caspase-3, 8, 9, and PARP in macrophages. Further, CTPS treatment reduced STS induced ROS production and MOMP increase in macrophages. In conclusion, CTPS treatment inhibits STS-induced macrophage apoptosis through inhibition of cytotoxicity, caspase activation, ROS production, and MOMP increase. These results suggest CTPS could be a potential candidate for treatment of immune cell protection effects.
Keywords: Cudrania tricuspidata, macrophage, apoptosis, protective roles
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