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JKFN Journal of the Korean Society of Food Science and Nutrition



Online ISSN 2288-5978

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Journal of the Korean Society of Food Science and Nutrition 2019; 48(12): 1330-1336

Published online December 31, 2019

Copyright © The Korean Society of Food Science and Nutrition.

Protective Roles of Polysaccharides Extracted from Cudrania tricuspidata Fruits in Macrophages

Eui-Baek Byun1, Kwangwook Kim2, Nam-Soon Oh2, Won-Jong Park2, Sang-Hyun Park2, Woo Sik Kim1, Ha-Yeon Song1, Jeong Moo Han1, and Eui-Hong Byun2

1Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute; 2Department of Food Science and Technology, Kongju National University

Abstract

It is believed that Cudrania tricuspidata fruits polysaccharides extracts (CTPS) have antioxidant, anti-inflammatory, anti-cancer, and immune activity effects. However, there have been no studies that have shown the cytoprotective effects of CTPS. Therefore, the present study was conducted to confirm the cytoprotective effect of CTPS in terms of their inhibition of cytotoxicity, caspase activity, reactive oxygen species (ROS) production, and mitochondrial outer membrane permeability (MOMP) increase in Raw264.7. Macrophage apoptosis was induced by staurosporine (STS) treatment. CTPS treatment significantly reduced STS induced cell death and annexin V positive cell levels in a dose-dependent manner. In addition, CTPS treatment inhibited activation of caspase-3, 8, 9, and PARP in macrophages. Further, CTPS treatment reduced STS induced ROS production and MOMP increase in macrophages. In conclusion, CTPS treatment inhibits STS-induced macrophage apoptosis through inhibition of cytotoxicity, caspase activation, ROS production, and MOMP increase. These results suggest CTPS could be a potential candidate for treatment of immune cell protection effects.

Keywords: Cudrania tricuspidata, macrophage, apoptosis, protective roles

Article

Article

Journal of the Korean Society of Food Science and Nutrition 2019; 48(12): 1330-1336

Published online December 31, 2019

Copyright © The Korean Society of Food Science and Nutrition.

꾸지뽕(Cudrania tricuspidata) 열매에서 추출된 조다당의 대식세포 보호 효과

Protective Roles of Polysaccharides Extracted from Cudrania tricuspidata Fruits in Macrophages

Eui-Baek Byun*1, Kwangwook Kim*2, Nam-Soon Oh*2, Won-Jong Park*2, Sang-Hyun Park*2, Woo Sik Kim*1, Ha-Yeon Song*1, Jeong Moo Han*1, and Eui-Hong Byun*2

*1Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute; *2Department of Food Science and Technology, Kongju National University

Abstract

It is believed that Cudrania tricuspidata fruits polysaccharides extracts (CTPS) have antioxidant, anti-inflammatory, anti-cancer, and immune activity effects. However, there have been no studies that have shown the cytoprotective effects of CTPS. Therefore, the present study was conducted to confirm the cytoprotective effect of CTPS in terms of their inhibition of cytotoxicity, caspase activity, reactive oxygen species (ROS) production, and mitochondrial outer membrane permeability (MOMP) increase in Raw264.7. Macrophage apoptosis was induced by staurosporine (STS) treatment. CTPS treatment significantly reduced STS induced cell death and annexin V positive cell levels in a dose-dependent manner. In addition, CTPS treatment inhibited activation of caspase-3, 8, 9, and PARP in macrophages. Further, CTPS treatment reduced STS induced ROS production and MOMP increase in macrophages. In conclusion, CTPS treatment inhibits STS-induced macrophage apoptosis through inhibition of cytotoxicity, caspase activation, ROS production, and MOMP increase. These results suggest CTPS could be a potential candidate for treatment of immune cell protection effects.

Keywords: Cudrania tricuspidata, macrophage, apoptosis, protective roles