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JKFN Journal of the Korean Society of Food Science and Nutrition



Online ISSN 2288-5978

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Journal of the Korean Society of Food Science and Nutrition 2018; 47(11): 1069-1075

Published online November 30, 2018

Copyright © The Korean Society of Food Science and Nutrition.

Immunomodulatory Activity of Platycodon grandiflorum, Codonopsis lanceolata, and Adenophora triphylla Extracts in Macrophage Cells

Eun-Hye Kim, Ji Young Gwak, and Mi Ja Chung

Department of Food Science and Nutrition, Gwangju University

Abstract

This study investigated the immunomodulatory and anti-inflammatory activities of water extract of Platycodon grandiflorum, Codonopsis lanceolata, and Adenophora triphylla (PG, CL, AT) in a murine RAW264.7 macrophage model. As immuno response biomarkers, the cell proliferation, nitric oxide (NO) production, tumor necrosis factor (TNF)-α production and degree of mitogen-activated protein kinase (MAPKs) phosphorylation in RAW264.7 macrophage cells were evaluated. PG and CL increased macrophage proliferation and production of NO and TNF-α, with greater immunostimulatory activities being observed in response to PG than CL in RAW264.7 cells. The increase of NO and TNF-α in PG or CL-treated cells was associated with phosphorylation of MAPKs, including extracellular signal-regulated kinase (ERK), p38 and c-Jun N-terminal kinase (JNK) in RAW264.7 cells. The phosphorylation of ERK, p38 and JNK (p-ERK, p-p38, p-JNK) of RAW264.7 cells treated with PG and CL, respectively, also increased significantly. The NO and TNF-α production and phosphorylation of ERK and p38 were unchanged in AT-treated RAW264.7 macrophages. Furthermore, we examined the effects of PG, CL and AT on NO and TNF-α production in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. PG, CL and AT inhibited LPS-induced NO and TNF-α production. These findings suggest that PG and CL can induce macrophage activation through MAPK signaling as immunomodulators and that they exert anti-inflammatory effects through inhibition of NO and TNF-α production.

Keywords: anti-inflammatory activity, Codonopsis lanceolata, immunomodulatory activity, macrophages, Platycodon grandiflorum

Article

Article

Journal of the Korean Society of Food Science and Nutrition 2018; 47(11): 1069-1075

Published online November 30, 2018

Copyright © The Korean Society of Food Science and Nutrition.

도라지, 더덕 그리고 잔대 추출물의 대식세포 면역조절 활성

Immunomodulatory Activity of Platycodon grandiflorum, Codonopsis lanceolata, and Adenophora triphylla Extracts in Macrophage Cells

Eun-Hye Kim, Ji Young Gwak, and Mi Ja Chung

Department of Food Science and Nutrition, Gwangju University

Abstract

This study investigated the immunomodulatory and anti-inflammatory activities of water extract of Platycodon grandiflorum, Codonopsis lanceolata, and Adenophora triphylla (PG, CL, AT) in a murine RAW264.7 macrophage model. As immuno response biomarkers, the cell proliferation, nitric oxide (NO) production, tumor necrosis factor (TNF)-α production and degree of mitogen-activated protein kinase (MAPKs) phosphorylation in RAW264.7 macrophage cells were evaluated. PG and CL increased macrophage proliferation and production of NO and TNF-α, with greater immunostimulatory activities being observed in response to PG than CL in RAW264.7 cells. The increase of NO and TNF-α in PG or CL-treated cells was associated with phosphorylation of MAPKs, including extracellular signal-regulated kinase (ERK), p38 and c-Jun N-terminal kinase (JNK) in RAW264.7 cells. The phosphorylation of ERK, p38 and JNK (p-ERK, p-p38, p-JNK) of RAW264.7 cells treated with PG and CL, respectively, also increased significantly. The NO and TNF-α production and phosphorylation of ERK and p38 were unchanged in AT-treated RAW264.7 macrophages. Furthermore, we examined the effects of PG, CL and AT on NO and TNF-α production in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. PG, CL and AT inhibited LPS-induced NO and TNF-α production. These findings suggest that PG and CL can induce macrophage activation through MAPK signaling as immunomodulators and that they exert anti-inflammatory effects through inhibition of NO and TNF-α production.

Keywords: anti-inflammatory activity, Codonopsis lanceolata, immunomodulatory activity, macrophages, Platycodon grandiflorum