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JKFN Journal of the Korean Society of Food Science and Nutrition



Online ISSN 2288-5978

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Journal of the Korean Society of Food Science and Nutrition 2018; 47(1): 15-23

Published online January 31, 2018

Copyright © The Korean Society of Food Science and Nutrition.

Cytoprotective Effect of Water Extract of Vitis amurensis Root against Oxidative Stress in HaCaT Human Keratinocytes

Eun Ok Choi1,2, Da Hye Kwon1,2, Hyun Hwang-Bo1,2, Jin-Woo Jeong1,2, Won Sok Nam3, Youn Hee Lee3, Hye Kyung Yang3, Hye Jin Hwang2,4, Byung Woo Kim2,5, Seung Hun Lee3, and Yung Hyun Choi1,2

1Open Laboratory for Muscular & Skeletal Disease Control and Department of Biochemistry, Dongeui University College of Korean Medicine; 2Anti-Aging Research Center and Blue Bio Industry RIC, 4Department of Food and Nutrition, College of Nursing, Health

Abstract

Root of Vitis amurensis Rupr., a wild-growing grape species, has been used as a traditional medicine for the treatment of various diseases in Asian countries, including Korea. Although several studies on the antioxidant effects of V. amurensis root extracts have been carried out, the underlying molecular mechanisms remain largely unresolved. In the present study, we investigated the cytoprotective efficacies of water extract of V. amurensis root (WEVA) against oxidative stress (hydrogen peroxide, H2O2) in HaCaT human keratinocytes. Our data show that treatment with WEVA prior to H2O2 exposure significantly increased HaCaT cell viability, indicating that exposure of HaCaT cells to WEVA conferred a protective effect against oxidative stress. WEVA preconditioning also effectively inhibited H2O2-induced apoptotic cell death by blocking degradation of poly (ADP-ribose) polymerase and down-regulation of pro-caspases-9 and -3. The anti-apoptotic effects of WEVA were associated with attenuation of mitochondrial dysfunction and normalization of expression changes of pro-apoptotic Bax and anti-apoptotic Bcl-2 in H2O2-stimulated HaCaT cells. In addition, WEVA abrogated H2O2-induced intracellular reactive oxygen species (ROS) accumulation and phosphorylation of histone H2AX at serine 139, a widely used marker of DNA damage. Moreover, WEVA increased the levels of hemeoxygenase-1 and NAD(P)H-quinone oxidoreductase-1, potent antioxidant enzymes associated with induction of nuclear transcription factor erythroid-2-like factor 2 (Nrf2). Thus, the present study demonstrates that WEVA protects human keratinocytes against oxidative stress-induced apoptosis and DNA damage, possibly through prevention of ROS generation and activation of Nrf2 signaling. Our data indicate that WEVA may potentially serve as an agent for treatment and prevention of skin disorders caused by oxidative stress.

Keywords: Vitis amurensis, antioxidant, apoptosis, ROS, DNA damage

Article

Article

Journal of the Korean Society of Food Science and Nutrition 2018; 47(1): 15-23

Published online January 31, 2018

Copyright © The Korean Society of Food Science and Nutrition.

HaCaT 인간 각질세포에서 산화적 스트레스에 대한 머루근 수용성 추출물의 세포 보호 효과

Cytoprotective Effect of Water Extract of Vitis amurensis Root against Oxidative Stress in HaCaT Human Keratinocytes

Eun Ok Choi*1*,*2, Da Hye Kwon*1*,*2, Hyun Hwang-Bo*1*,*2, Jin-Woo Jeong*1*,*2, Won Sok Nam*3, Youn Hee Lee*3, Hye Kyung Yang*3, Hye Jin Hwang*2*,*4, Byung Woo Kim*2*,*5, Seung Hun Lee*3, and Yung Hyun Choi*1*,*2

*1Open Laboratory for Muscular & Skeletal Disease Control and Department of Biochemistry, Dongeui University College of Korean Medicine; *2Anti-Aging Research Center and Blue Bio Industry RIC, *4Department of Food and Nutrition, College of Nursing, Health

Abstract

Root of Vitis amurensis Rupr., a wild-growing grape species, has been used as a traditional medicine for the treatment of various diseases in Asian countries, including Korea. Although several studies on the antioxidant effects of V. amurensis root extracts have been carried out, the underlying molecular mechanisms remain largely unresolved. In the present study, we investigated the cytoprotective efficacies of water extract of V. amurensis root (WEVA) against oxidative stress (hydrogen peroxide, H2O2) in HaCaT human keratinocytes. Our data show that treatment with WEVA prior to H2O2 exposure significantly increased HaCaT cell viability, indicating that exposure of HaCaT cells to WEVA conferred a protective effect against oxidative stress. WEVA preconditioning also effectively inhibited H2O2-induced apoptotic cell death by blocking degradation of poly (ADP-ribose) polymerase and down-regulation of pro-caspases-9 and -3. The anti-apoptotic effects of WEVA were associated with attenuation of mitochondrial dysfunction and normalization of expression changes of pro-apoptotic Bax and anti-apoptotic Bcl-2 in H2O2-stimulated HaCaT cells. In addition, WEVA abrogated H2O2-induced intracellular reactive oxygen species (ROS) accumulation and phosphorylation of histone H2AX at serine 139, a widely used marker of DNA damage. Moreover, WEVA increased the levels of hemeoxygenase-1 and NAD(P)H-quinone oxidoreductase-1, potent antioxidant enzymes associated with induction of nuclear transcription factor erythroid-2-like factor 2 (Nrf2). Thus, the present study demonstrates that WEVA protects human keratinocytes against oxidative stress-induced apoptosis and DNA damage, possibly through prevention of ROS generation and activation of Nrf2 signaling. Our data indicate that WEVA may potentially serve as an agent for treatment and prevention of skin disorders caused by oxidative stress.

Keywords: Vitis amurensis, antioxidant, apoptosis, ROS, DNA damage