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JKFN Journal of the Korean Society of Food Science and Nutrition



Online ISSN 2288-5978

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Journal of the Korean Society of Food Science and Nutrition 2017; 46(5): 537-544

Published online May 31, 2017

Copyright © The Korean Society of Food Science and Nutrition.

Anti-Inflammatory Effect of Ethanolic Extract from Polyopes affinis through Suppression of NF-κB and MAPK Activation in LPS-Stimulated RAW 264.7 Cells

Min-Ji Kim1, Koth-Bong-Woo-Ri Kim1, Sun-Hee Park1, So-Young Park1, Hyeun-Deok Choi2, Jung-Su Choi3, Mi-Ran Jang4, Moo-Hyeog Im5, and Dong-Hyun Ahn1

1Department of Food Science and Tehcnology/Institute of Food Sciences and 2Institute of Fisheries Sciences, Pukyong National University; 3Subdivision of Culinary Arts, Kyungnam College of Information and Technology; 4Health Functional Food Policy Divi

Abstract

In this study, the anti-inflammatory effect of Polyopes affinis ethanol extract (PAEE) was investigated using LPS-stimulated RAW 264.7 cells and a croton oil-induced ICR mice model. Treatment with PAEE significantly reduced production of nitric oxide (NO) and pro-inflammatory cytokines [interleukin (IL)-6, tumor necrosis factor (TNF)-α, and IL-1β] in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. PAEE treatment also reduced expression of inducible NO synthase, cyclooxygenase-2, nuclear factor-κB, and mitogen-activated protein kinases in LPS-stimulated RAW 264.7 cells. In the croton oil-induced ear edema test, application of PAEE (10∼250 mg/kg body weight) reduced ear edema in a dose-dependent manner, and PAEE treatment at 50 mg/kg body weight showed similar inhibitory effects compared with prednisolone (10 mg/kg body weight). Histological analysis revealed reduced dermal thickness and lower number of infiltrated mast cells. These results suggest that PAEE might be used as a promising anti-inflammatory agent for inhibition of LPS-induced inflammation and ear edema formation.

Keywords: Polyopes affinis, anti-inflammatory effect, NF-κB, MAPKs, ear edema

Article

Article

Journal of the Korean Society of Food Science and Nutrition 2017; 46(5): 537-544

Published online May 31, 2017

Copyright © The Korean Society of Food Science and Nutrition.

LPS로 자극된 대식세포에서의 NF-κB와 MAPK 활성 조절을 통한 참까막살(Polyopes affinis) 에탄올 추출물의 항염증 효과

Anti-Inflammatory Effect of Ethanolic Extract from Polyopes affinis through Suppression of NF-κB and MAPK Activation in LPS-Stimulated RAW 264.7 Cells

Min-Ji Kim*1, Koth-Bong-Woo-Ri Kim*1, Sun-Hee Park*1, So-Young Park*1, Hyeun-Deok Choi*2, Jung-Su Choi*3, Mi-Ran Jang*4, Moo-Hyeog Im*5, and Dong-Hyun Ahn*1

*1Department of Food Science and Tehcnology/Institute of Food Sciences and *2Institute of Fisheries Sciences, Pukyong National University; *3Subdivision of Culinary Arts, Kyungnam College of Information and Technology; *4Health Functional Food Policy Divi

Abstract

In this study, the anti-inflammatory effect of Polyopes affinis ethanol extract (PAEE) was investigated using LPS-stimulated RAW 264.7 cells and a croton oil-induced ICR mice model. Treatment with PAEE significantly reduced production of nitric oxide (NO) and pro-inflammatory cytokines [interleukin (IL)-6, tumor necrosis factor (TNF)-α, and IL-1β] in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. PAEE treatment also reduced expression of inducible NO synthase, cyclooxygenase-2, nuclear factor-κB, and mitogen-activated protein kinases in LPS-stimulated RAW 264.7 cells. In the croton oil-induced ear edema test, application of PAEE (10∼250 mg/kg body weight) reduced ear edema in a dose-dependent manner, and PAEE treatment at 50 mg/kg body weight showed similar inhibitory effects compared with prednisolone (10 mg/kg body weight). Histological analysis revealed reduced dermal thickness and lower number of infiltrated mast cells. These results suggest that PAEE might be used as a promising anti-inflammatory agent for inhibition of LPS-induced inflammation and ear edema formation.

Keywords: Polyopes affinis, anti-inflammatory effect, NF-κB, MAPKs, ear edema