This study investigated the effects of Sinetrol-XPur (polyphenolic Citrus spp. and Paullinia cupana Kunth dry extract) on lipolysis using leptin-deficient obese (ob/ob) mice. Obese mice were treated with two different doses, 100 mg/kg body weight (B.W.) and 300 mg/kg B.W. in each AIN93G supplement, for 7 weeks. Body weight gain in obese mice treated with both low and high doses of Sinetrol-XPur was reduced compared with control obese mice. Abdominal and visceral adipose tissue weight of mice were reduced in high dose supplemented groups. Epididymal adipose tissue weight was reduced in both low and high dose supplemented groups by 18.27% and 41.05%, respectively. Phosphodiesterase 3B (PDE3B) mRNA levels decreased upon Sinetrol supplementation in adipose tissue of ob/ob mice, whereas A kinase anchor protein 1 (AKAP1), adipose triglyceride lipase (ATGL), and perilipin (PLIN) mRNA levels increased. These results suggest that Sinetrol-XPur supplementation partially stimulates lipolysis through reduction of PDE3B and induction of AKAP1, ATGL, and/or PLIN gene expression, resulting in reduced body and white adipose tissue weight.

Keywords: lipolysis, Sinetrol-XPur, citrus, ob/ob mice