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JKFN Journal of the Korean Society of Food Science and Nutrition



Online ISSN 2288-5978

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Journal of the Korean Society of Food Science and Nutrition 2017; 46(2): 169-176

Published online February 28, 2017

Copyright © The Korean Society of Food Science and Nutrition.

Effect of Tartary Buckwheat Sprout on Non-Alcoholic Fatty Liver Disease through Anti-Histone Acetyltransferase Activity

Jin-Taek Hwang1,2, Tae Gyu Nam1, Min-Yu Chung1, Jae Ho Park1,2, and Hyo-Kyoung Choi1

1Korea Food Research Institute; 2Korea University of Science & Technology

Abstract

Non-alcoholic fatty liver disease (NAFLD) is caused by chronic lipid accumulation due to dysregulation of lipid metabolism in the liver, and it is associated with various human diseases such as obesity, dyslipidemia, hypertension, and diabetes. Histone acetylation is a representative epigenetic mechanism regulated by histone acetyltransferases (HATs) and deacetylases. We observed that tartary buckwheat sprout (TBS) suppressed lipid accumulation in HepG2 cells through its anti-HAT activity. We showed that TBS was a novel HAT inhibitor with specificity for the major HAT enzyme p300. Importantly, TBS reduced acetylation of total and histone proteins, H3K9, H3K36, and H4K8, resulting in decreased transcriptional activities of sterol regulatory element-binding protein 1c, ATP citrate lyase, and fatty acid synthase. These results suggest that TBS inhibits the NAFLD transcription-modulating activity of lipogenesis-related genes through modification of histone acetylation.

Keywords: non-alcoholic fatty liver disease, histone acetyltransferase, lipid accumulation, HepG2, tartary buckwheat sprout

Article

Article

Journal of the Korean Society of Food Science and Nutrition 2017; 46(2): 169-176

Published online February 28, 2017

Copyright © The Korean Society of Food Science and Nutrition.

쓴메밀 새싹 추출물의 히스톤 아세틸화 효소 활성 저해에 의한 비알코올성 지방간 억제 효능

Effect of Tartary Buckwheat Sprout on Non-Alcoholic Fatty Liver Disease through Anti-Histone Acetyltransferase Activity

Jin-Taek Hwang*1*,*2, Tae Gyu Nam*1, Min-Yu Chung*1, Jae Ho Park*1*,*2, and Hyo-Kyoung Choi*1

*1Korea Food Research Institute; *2Korea University of Science & Technology

Abstract

Non-alcoholic fatty liver disease (NAFLD) is caused by chronic lipid accumulation due to dysregulation of lipid metabolism in the liver, and it is associated with various human diseases such as obesity, dyslipidemia, hypertension, and diabetes. Histone acetylation is a representative epigenetic mechanism regulated by histone acetyltransferases (HATs) and deacetylases. We observed that tartary buckwheat sprout (TBS) suppressed lipid accumulation in HepG2 cells through its anti-HAT activity. We showed that TBS was a novel HAT inhibitor with specificity for the major HAT enzyme p300. Importantly, TBS reduced acetylation of total and histone proteins, H3K9, H3K36, and H4K8, resulting in decreased transcriptional activities of sterol regulatory element-binding protein 1c, ATP citrate lyase, and fatty acid synthase. These results suggest that TBS inhibits the NAFLD transcription-modulating activity of lipogenesis-related genes through modification of histone acetylation.

Keywords: non-alcoholic fatty liver disease, histone acetyltransferase, lipid accumulation, HepG2, tartary buckwheat sprout