Ex) Article Title, Author, Keywords
Online ISSN 2288-5978
Ex) Article Title, Author, Keywords
Journal of the Korean Society of Food Science and Nutrition 2017; 46(2): 169-176
Published online February 28, 2017
Copyright © The Korean Society of Food Science and Nutrition.
Jin-Taek Hwang1,2, Tae Gyu Nam1, Min-Yu Chung1, Jae Ho Park1,2, and Hyo-Kyoung Choi1
1Korea Food Research Institute; 2Korea University of Science & Technology
Non-alcoholic fatty liver disease (NAFLD) is caused by chronic lipid accumulation due to dysregulation of lipid metabolism in the liver, and it is associated with various human diseases such as obesity, dyslipidemia, hypertension, and diabetes. Histone acetylation is a representative epigenetic mechanism regulated by histone acetyltransferases (HATs) and deacetylases. We observed that tartary buckwheat sprout (TBS) suppressed lipid accumulation in HepG2 cells through its anti-HAT activity. We showed that TBS was a novel HAT inhibitor with specificity for the major HAT enzyme p300. Importantly, TBS reduced acetylation of total and histone proteins, H3K9, H3K36, and H4K8, resulting in decreased transcriptional activities of sterol regulatory element-binding protein 1c, ATP citrate lyase, and fatty acid synthase. These results suggest that TBS inhibits the NAFLD transcription-modulating activity of lipogenesis-related genes through modification of histone acetylation.
Keywords: non-alcoholic fatty liver disease, histone acetyltransferase, lipid accumulation, HepG2, tartary buckwheat sprout
Journal of the Korean Society of Food Science and Nutrition 2017; 46(2): 169-176
Published online February 28, 2017
Copyright © The Korean Society of Food Science and Nutrition.
Jin-Taek Hwang*1*,*2, Tae Gyu Nam*1, Min-Yu Chung*1, Jae Ho Park*1*,*2, and Hyo-Kyoung Choi*1
*1Korea Food Research Institute; *2Korea University of Science & Technology
Non-alcoholic fatty liver disease (NAFLD) is caused by chronic lipid accumulation due to dysregulation of lipid metabolism in the liver, and it is associated with various human diseases such as obesity, dyslipidemia, hypertension, and diabetes. Histone acetylation is a representative epigenetic mechanism regulated by histone acetyltransferases (HATs) and deacetylases. We observed that tartary buckwheat sprout (TBS) suppressed lipid accumulation in HepG2 cells through its anti-HAT activity. We showed that TBS was a novel HAT inhibitor with specificity for the major HAT enzyme p300. Importantly, TBS reduced acetylation of total and histone proteins, H3K9, H3K36, and H4K8, resulting in decreased transcriptional activities of sterol regulatory element-binding protein 1c, ATP citrate lyase, and fatty acid synthase. These results suggest that TBS inhibits the NAFLD transcription-modulating activity of lipogenesis-related genes through modification of histone acetylation.
Keywords: non-alcoholic fatty liver disease, histone acetyltransferase, lipid accumulation, HepG2, tartary buckwheat sprout
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