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JKFN Journal of the Korean Society of Food Science and Nutrition



Online ISSN 2288-5978

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Journal of the Korean Society of Food Science and Nutrition 2017; 46(11): 1271-1277

Published online November 30, 2017

Copyright © The Korean Society of Food Science and Nutrition.

Antioxidant and Anti-Inflammatory Effects of Kamisipjeondaebotang in RAW 264.7 Cells

Jeong-Ho Myung and Myung-Sun Lee

Department of Health and Beauty, Daejeon University

Abstract

As Kamisipjeondaebotang (KSD) extract is an herbal ingredient, safety is very important due to possible cell poisoning or heavy metal toxicity to organs when administered to humans or animals. Accordingly, this study examined the antioxidant and anti-inflammatory effects of KSD extract to confirm its medicinal safety by using RAW 264.7 cells after heavy metal screening, functional index test of the liver and kidney, and cell survival rate test. Heavy metals were not found in KSD extracts or were less than standard amounts. Liver function indices such as aspartate aminotransferase and alanine aminotransferase revealed low values and kidney function indices such as creatinine and blood urea nitrogen were not significantly different from the normal group. This proved the safety to the human. RAW 264.7 cells showed no poisoning compared to the control group in terms of survival rate. Regarding the antioxidant effect of KSD extract, 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) radical scavenging activity increased at concentrations over 10 μg/mL. The anti-inflammatory effect of KSD extract significantly decreased based on the amount of nitric oxide at concentrations of 10 and 100 μg/mL compared to the control group. Expression of interleukin (IL)-1β and IL-6 decreased in a concentration-dependent manner. There was no significant difference in tumor necrosis factor-α level. Based on the results, KSD can be regarded as a safe antioxidant with anti-inflammatory effects for fracture treatment.

Keywords: anti-inflammatory effect, pro-inflammatory cytokines, Kamisipjeondaebotang

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Article

Journal of the Korean Society of Food Science and Nutrition 2017; 46(11): 1271-1277

Published online November 30, 2017

Copyright © The Korean Society of Food Science and Nutrition.

가미십전대보탕의 RAW 264.7 세포에서 항산화 및 항염증 효과

Antioxidant and Anti-Inflammatory Effects of Kamisipjeondaebotang in RAW 264.7 Cells

Jeong-Ho Myung and Myung-Sun Lee

Department of Health and Beauty, Daejeon University

Abstract

As Kamisipjeondaebotang (KSD) extract is an herbal ingredient, safety is very important due to possible cell poisoning or heavy metal toxicity to organs when administered to humans or animals. Accordingly, this study examined the antioxidant and anti-inflammatory effects of KSD extract to confirm its medicinal safety by using RAW 264.7 cells after heavy metal screening, functional index test of the liver and kidney, and cell survival rate test. Heavy metals were not found in KSD extracts or were less than standard amounts. Liver function indices such as aspartate aminotransferase and alanine aminotransferase revealed low values and kidney function indices such as creatinine and blood urea nitrogen were not significantly different from the normal group. This proved the safety to the human. RAW 264.7 cells showed no poisoning compared to the control group in terms of survival rate. Regarding the antioxidant effect of KSD extract, 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) radical scavenging activity increased at concentrations over 10 μg/mL. The anti-inflammatory effect of KSD extract significantly decreased based on the amount of nitric oxide at concentrations of 10 and 100 μg/mL compared to the control group. Expression of interleukin (IL)-1β and IL-6 decreased in a concentration-dependent manner. There was no significant difference in tumor necrosis factor-α level. Based on the results, KSD can be regarded as a safe antioxidant with anti-inflammatory effects for fracture treatment.

Keywords: anti-inflammatory effect, pro-inflammatory cytokines, Kamisipjeondaebotang