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JKFN Journal of the Korean Society of Food Science and Nutrition



Online ISSN 2288-5978

Article

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Journal of the Korean Society of Food Science and Nutrition 2012; 41(3): 310-319

Published online March 31, 2012

Copyright © The Korean Society of Food Science and Nutrition.

Effects of Bovine α-Lactalbumin Added with Oleic Acid and Microbial Transglutaminase on Cancer Cell Apoptosis

Ji-Eun Jeong and Youn-Ho Hong

Dept. of Food and Nutrition․Human Ecology Research Institute, College of Human Ecology, Chonnam National University, Gwangju 500-757, Korea

Abstract

In order to investigate the biological effects of conformational changes in the folding state of bovine α-lactalbumin (α-La), the protein was prepared and classified as apo form, microbial transglutaminase (MTGase) added form, or bovine α-La made lethal to tumor cell (BAMLET) form. Apo α-La form showed weaker cancer cell inhibitory activity (apoptosis) than native α-La, which suggests that the metal ion-like Ca2+ had a positive effect, whereas BAMLET form showed strong cancer cell apoptotic activity. The BAMLET form seemed to be a molten globule structure that increased hydrophobicity. MTGase added to apo α-La polymer showed similar anti-cancer activity as native α-La, and it was well hydrolyzed by digestive enzymes. NMR results showed that BAMLET interacted with oleic acid and produced a complex.

Keywords: bovine α-lactalbumin, oleic acid, tranglutaminase, apoptosis

Article

Journal of the Korean Society of Food Science and Nutrition 2012; 41(3): 310-319

Published online March 31, 2012

Copyright © The Korean Society of Food Science and Nutrition.

올레산과 미생물 유래 트랜스글루타미나제를 첨가한 우유 알파-락트알부민의 암세포사멸 효과

Effects of Bovine α-Lactalbumin Added with Oleic Acid and Microbial Transglutaminase on Cancer Cell Apoptosis

Ji-Eun Jeong and Youn-Ho Hong

Dept. of Food and Nutrition․Human Ecology Research Institute, College of Human Ecology, Chonnam National University, Gwangju 500-757, Korea

Abstract

In order to investigate the biological effects of conformational changes in the folding state of bovine α-lactalbumin (α-La), the protein was prepared and classified as apo form, microbial transglutaminase (MTGase) added form, or bovine α-La made lethal to tumor cell (BAMLET) form. Apo α-La form showed weaker cancer cell inhibitory activity (apoptosis) than native α-La, which suggests that the metal ion-like Ca2+ had a positive effect, whereas BAMLET form showed strong cancer cell apoptotic activity. The BAMLET form seemed to be a molten globule structure that increased hydrophobicity. MTGase added to apo α-La polymer showed similar anti-cancer activity as native α-La, and it was well hydrolyzed by digestive enzymes. NMR results showed that BAMLET interacted with oleic acid and produced a complex.

Keywords: bovine α-lactalbumin, oleic acid, tranglutaminase, apoptosis