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JKFN Journal of the Korean Society of Food Science and Nutrition



Online ISSN 2288-5978

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Journal of the Korean Society of Food Science and Nutrition 2020; 49(11): 1202-1211

Published online November 30, 2020

Copyright © The Korean Society of Food Science and Nutrition.

Anti-Inflammatory Effects of BENDU381 in Lipopolysaccharide-Stimulated RAW264.7 Cells

Jin A. Shin1 and Jong-Moon Jeong2

1Biotechnology Research Center, Ben’s Lab Co., Ltd. 2Department of Bioscience, The University of Suwon

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

BENDU381, a nutraceutical composed of extracts from green tea (Camellia sinensis), guajava leaves (Psidium guajava) and rose petals (Rosa hybrida), has been previously shown to improve allergic inflammation and has also been evaluated for its therapeutic effect on allergic rhinitis. In this study, we examined the anti-inflammatory effect of BENDU381 on lipopolysaccharide (LPS)-stimulated mouse macrophage, RAW264.7 cells, and the molecular mechanisms responsible for its anti-inflammatory activity. BENDU381 significantly suppressed the expression of interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α, as well as cyclooxygenase (COX)-2 and inducible nitric oxide synthase (iNOS) I the LPS-stimulated RAW264.7 cells. Also, BENDU381 inhibited LPS-induced activation of nuclear factor (NF)-κB by dampening the activation of the mitogen-activated protein kinases (MAPKs) pathway including c-Jun N-terminal kinase (JNK) and extracellular-signal-regulated kinase (ERK) which are responsible for the expression of pro-inflammatory mediators. These results suggest that BENDU381 has strong anti-inflammatory activities and its effect in the mouse macrophages is mediated by the blockage of NF-κB-JNK/ERK MAPKs signaling pathway.

Keywords: BENDU381, anti-inflammatory, macrophage, cytokines, MAPKs

Article

Article

Journal of the Korean Society of Food Science and Nutrition 2020; 49(11): 1202-1211

Published online November 30, 2020

Copyright © The Korean Society of Food Science and Nutrition.

LPS를 이용한 염증유도 RAW264.7 세포에서 BENDU381의 항염 효과 연구

Anti-Inflammatory Effects of BENDU381 in Lipopolysaccharide-Stimulated RAW264.7 Cells

Jin A. Shin*1 and Jong-Moon Jeong*2

*1Biotechnology Research Center, Ben’s Lab Co., Ltd. *2Department of Bioscience, The University of Suwon

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

BENDU381, a nutraceutical composed of extracts from green tea (Camellia sinensis), guajava leaves (Psidium guajava) and rose petals (Rosa hybrida), has been previously shown to improve allergic inflammation and has also been evaluated for its therapeutic effect on allergic rhinitis. In this study, we examined the anti-inflammatory effect of BENDU381 on lipopolysaccharide (LPS)-stimulated mouse macrophage, RAW264.7 cells, and the molecular mechanisms responsible for its anti-inflammatory activity. BENDU381 significantly suppressed the expression of interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α, as well as cyclooxygenase (COX)-2 and inducible nitric oxide synthase (iNOS) I the LPS-stimulated RAW264.7 cells. Also, BENDU381 inhibited LPS-induced activation of nuclear factor (NF)-κB by dampening the activation of the mitogen-activated protein kinases (MAPKs) pathway including c-Jun N-terminal kinase (JNK) and extracellular-signal-regulated kinase (ERK) which are responsible for the expression of pro-inflammatory mediators. These results suggest that BENDU381 has strong anti-inflammatory activities and its effect in the mouse macrophages is mediated by the blockage of NF-κB-JNK/ERK MAPKs signaling pathway.

Keywords: BENDU381, anti-inflammatory, macrophage, cytokines, MAPKs